The separation of cells and organelles by partitioning in two-polymer aqueous phases

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The separation of cells and organelles by partitioning in two-polymer aqueous phases.

When solutions of poly(ethylene glycol) and dextran are mixed above certain concentrations, they form two immiscible aqueous phases: a poly(ethylene glycol)-rich top phase and a dextranrich bottom phase. These solutions can be buffered and made isotonic. Cells and organelles added to such systems will partition between the two phases and the horizontal interface between them. Depending on the c...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1981

ISSN: 0264-6021

DOI: 10.1042/bj1960001